H E Staining Protocol Paraffin Sections

Ethanol 100 5 min.

H e staining protocol paraffin sections. Xylene desp 20 min. Ethanol 100 5 min. Use of very cold water slows down the.

Wash in running tap water for 5 minutes. 2 recommendations for deparaffinization. Deparaffinize sections 2 changes of xylene 10 minutes each.

The staining procedure for h e follows a basic protocol. Dewaxing dehydration hematoxylin differentiation bluing eosin dehydration clearing cover slipping. 3 x 3 xylene blot excess xylene before going into ethanol 3 x 3 100 ethanol 1 x 3 95 ethanol 1 x 3 80 ethanol 1 x 5 deionized h2o while sections are in water skim surface of hematoxalin with a kimwipe to remove oxidized particles.

1 p a g e h e staining paraffin embedded sections 1. Allow the slides to dry overnight and store slides at room temperature until ready for use. Use three changes of xylene 3.

Transfer the sections onto a superfrost plus slide. A few drops of strong ammonium hydroxide or of saturated aqueous lithium carbonate added immediately before use are sufficient for a 400 ml staining dish full of water. Section paraffin blocks at the desired thickness usually 4 5 µm on a microtome and float on a 40 c water bath containing distilled water.

If paraffin is not totally removed from tissue sections color intensity may be decreased or staining may be irregular spotty within the tissue section. Stain in harris hematoxylin solution for 8 minutes. Thus the stain discloses abundant structural information with specific functional implications.